Activation of the Nrf2 Antioxidant Pathway by Longjing Green Tea Polyphenols in Mice Livers

Previous studies have revealed that green tea polyphenol (GTP) could protect against liver injury due to oxidative stress. However, the mechanism underlying bioactive actions of GTP in has not been systematically evaluated. This study aimed investigate effect on activation nuclear factor erythroid-2-related 2 (Nrf2)-Kelch-like ECH-associated protein 1 (keap1) pathway, using silico and vivo methods. Furthermore, regulation Nrf2 downstream target antioxidant response element (ARE) was also The high-performance liquid chromatography analysis indicated includes 9 major compounds, molecule docking demonstrated most these polyphenols a strong binding affinity with keap1 Kelch domain, where binds Neh2 domain Nrf2. Remarkably, predominant compound GTP, is, epigallocatechin gallate, displayed best score, which can fully occupy all 3 polar subpockets domain. Nrf2, keap1, gene expression levels were changed livers compared control group. It showed level significantly upregulated GTP-induced mice across treatments, while remained unchanged. Subsequently, we observed significant increasing trend ARE, including antioxidative enzymes, phase II efflux transporters livers. present activate signaling pathway by interrupting Nrf2-keap1 protein–protein interaction